Palmitate-induced changes in protein expression of insulin secreting INS-1E cells
2010 (English)In: Journal of Proteomics, ISSN 1876-7737, Vol. 73, no 6, 1148-1155 p.Article in journal (Refereed) Published
Elevated blood levels of glucose and lipids in individuals with type 2 diabetes mellitus have been observed to cause impairment of insulin secretion from pancreatic beta-cells. Chronic exposure to either of the circulating fatty acid oleate or palmitate has different effects on the beta-cell. Whereas palmitate causes functional impairment of the beta-cell and apoptosis, oleate has only minor negative effects on beta-cell function and mass. The aim of the present study was to delineate mechanisms by which the fatty acids affect the beta-cell differently. In particular, the aim was to identify beta-cell proteins exclusively regulated by palmitate. INS-1E cells were cultured for 24 h in medium supplemented with palmitate or oleate. After culture, cells were lysed and subjected to two-dimensional gel electrophoresis. Proteins specifically regulated by palmitate were excised from the gel and identified by peptide mass fingerprinting using MALDI-TOF MS. Proteins exclusively regulated by palmitate were classified into proteins of carbohydrate or protein metabolism and Ca2+ or mRNA binding proteins. The specific palmitate-induced down-regulation of enzymes of glycolysis, proteins of protein turnover and anti-apoptotic protein may contribute to explain the different effects exerted by palmitate and oleate on beta-cell function and mass.
Place, publisher, year, edition, pages
2010. Vol. 73, no 6, 1148-1155 p.
Two-dimensional gel electrophoresis, beta-cell, Lipotoxicity, Diabetes, Oleate, Palmitate, Insulin
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-136894DOI: 10.1016/j.jprot.2010.01.012ISI: 000277763800012OAI: oai:DiVA.org:uu-136894DiVA: diva2:377746