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Essential tactics of tissue preparation and matrix nano-spotting for successful compound imaging mass spectrometry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
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2010 (English)In: Journal of Proteomics, ISSN 1876-7737, Vol. 73, no 6, 1270-1278 p.Article in journal (Refereed) Published
Abstract [en]

The ultimate goal of MALDI-Imaging Mass Spectrometry (MALDI-IMS) is to achieve spatial localization of analytes in tissue sections down to individual tissue compartments or even at the level of a few cells. With compound tissue imaging, it is possible to track the transportation of an unlabelled, inhaled reference compound within lung tissue, through the application of MALDI-IMS. The procedure for isolation and preparation of lung tissues is found to be crucial in order to preserve the anatomy and structure of the pulmonary compartments. To avoid delocalization of analytes within lung tissue compartments we have applied an in-house designed nano-spotter, based on a microdispenser mounted on an XY table, of which movement and spotting functionality were fully computer controlled. We demonstrate the usefulness of this platform in lung tissue sections isolated from rodent in vivo model, applied to compound tissue imaging as exemplified with the determination of the spatial distribution of (1 alpha,2 beta,4 beta,7 beta)-7-[(hydroxidi-2-thienylacetyl)oxy]-9,9-dimethyl-3-oxa-9-azoniatric yclo[,4)]nonane, also known as tiotropium. We provide details on tissue preparation protocols and sample spotting technology for successful identification of drug in mouse lung tissue by using MALDI-Orbitrap instrumentation.

Place, publisher, year, edition, pages
2010. Vol. 73, no 6, 1270-1278 p.
Keyword [en]
Tissue-imaging MALDI-MS, Microdispenser, Matrix spotting, Rat lung, Tiotropium, MALDI Orbitrap
National Category
Pharmaceutical Sciences
URN: urn:nbn:se:uu:diva-136895DOI: 10.1016/j.jprot.2010.02.021ISI: 000277763800023OAI: oai:DiVA.org:uu-136895DiVA: diva2:377751
Available from: 2010-12-14 Created: 2010-12-14 Last updated: 2010-12-14Bibliographically approved

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