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Selection of candidate coding DNA barcoding regions for use on land plants
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2009 (English)In: Botanical journal of the Linnean Society, ISSN 0024-4074, E-ISSN 1095-8339, Vol. 159, no 1, 1-11 p.Article in journal (Refereed) Published
Abstract [en]

An in silico screen of 41 of the 81 coding regions of the Nicotiana plastid genome generated a shortlist of 12 candidates as DNA barcoding loci for land plants. These loci were evaluated for amplification and sequence variation against a reference set of 98 land plant taxa. The deployment of multiple primers and a modified multiplexed tandem polymerase chain reaction yielded 85-94% amplification across taxa, and mean sequence differences between sister taxa of 6.1 from 156 bases of accD to 22 from 493 bases of matK. We conclude that loci should be combined for effective diagnosis, and recommend further investigation of the following six loci: matK, rpoB, rpoC1, ndhJ, ycf5 and accD.

Place, publisher, year, edition, pages
2009. Vol. 159, no 1, 1-11 p.
Keyword [en]
genetic barcodes, matK, MT-PCR, multi-locus barcode, plant identification, plastid genes
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-137347DOI: 10.1111/j.1095-8339.2008.00938.xISI: 000262509300001OAI: oai:DiVA.org:uu-137347DiVA: diva2:378176
Available from: 2010-12-15 Created: 2010-12-15 Last updated: 2017-12-11Bibliographically approved

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