uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Expression and association of TRPC subtypes with Orai1 and STIM1 in human parathyroid
Show others and affiliations
2010 (English)In: Journal of Molecular Endocrinology, ISSN 0952-5041, Vol. 44, no 5, 285-294 p.Article in journal (Refereed) Published
Abstract [en]

The mechanism behind Ca2+ entry into the parathyroid cells has been widely debated, and the molecular identities of the responsible ion channels have not been established yet. In this study, we show that the parathyroid cells lack voltage-operated Ca2+ channels. Passive store depletion by thapsigargin, on the other hand, induces a large non-voltage-activated non-selective cation current. The increase in intracellular Ca2+ caused by thapsigargin is attenuated by 2-aminoethoxydiphenyl borate, a blocker of store-operated Ca2+ entry (SOCE). Candidate molecules for non-voltage-operated Ca2+ signaling were investigated. These included members of the transient receptor potential canonical (TRPC) ion channel family, as well as Ca2+ release-activated Ca2+ modulator 1 (Orai1) and stromal interaction molecule 1 (STIM1) that are key proteins in the SOCE pathway. Using RT-PCR screening, quantitative real-time PCR, and western blot, we showed expression of TRPC1, TRPC4, and TRPC6; Orai1; and STIM1 genes and proteins in normal and adenomatous human parathyroid tissues. Furthermore, co-immunoprecipitation experiments demonstrated a ternary complex of TRPC1-Orai1-STIM1, supporting a physical interaction between these molecules in human parathyroid.

Place, publisher, year, edition, pages
2010. Vol. 44, no 5, 285-294 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-137063DOI: 10.1677/JME-09-0138ISI: 000276533300003PubMedID: 20194530OAI: oai:DiVA.org:uu-137063DiVA: diva2:378409
Available from: 2010-12-15 Created: 2010-12-15 Last updated: 2011-03-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Endocrine Surgery
In the same journal
Journal of Molecular Endocrinology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 123 hits
ReferencesLink to record
Permanent link

Direct link