Global and unbiased detection of splice junctions from RNA-seq data
2010 (English)In: Genome Biology, ISSN 1474-760X, Vol. 11, no 3, R34- p.Article in journal (Refereed) Published
We have developed a new strategy for de novo prediction of splice junctions in short-read RNA-seq data, suitable for detection of novel splicing events and chimeric transcripts. When tested on mouse RNA-seq data, > 31,000 splice events were predicted, of which 88% bridged between two regions separated by <= 100 kb, and 74% connected two exons of the same RefSeq gene. Our method also reports genomic rearrangements such as insertions and deletions.
Place, publisher, year, edition, pages
2010. Vol. 11, no 3, R34- p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-137747DOI: 10.1186/gb-2010-11-3-r34ISI: 000277309100016OAI: oai:DiVA.org:uu-137747DiVA: diva2:378865