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Giardia intestinalis: Aphidicolin influence on the trophozoite cell cycle
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
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2010 (English)In: Experimental parasitology, ISSN 0014-4894, E-ISSN 1090-2449, Vol. 124, no 2, 159-166 p.Article in journal (Refereed) Published
Abstract [en]

This study is a thorough examination of the effects of the DNA polymerase inhibitor aphidicolin on the nuclear cycle and cell cycle progression characteristics, as well as their reversibility, in Giardia intestinalis. Giardia trophozoites are arrested in the G1/S-junction after aphidicolin treatment according to their DNA content. However, cell growth continues and trophozoites arrested with aphidicolin resemble cells in the G2 phase and trophozoites in ageing cultures. Extensive treatment with aphidicolin causes side effects and we detected positive signals for phosphorylated histone H2A, which, in mammalian cells. is involved in a signalling pathway triggered as a reaction to double stranded DNA breaks. These results suggest that aphidicolin causes dissociation of the nuclear and cytoplasmic cycles, a phenomenon that has also been described for other inhibitors in mammalian cell lines. Thus, if aphidicolin is used for synchronization of Giardia trophozoites, this fact must be accounted for, and treatment with aphidicolin must be minimal. (C) 2009 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2010. Vol. 124, no 2, 159-166 p.
Keyword [en]
Giardia intestinalis, Diplomonad, Aphidicolin, Nuclear cycle, Cytoplasmic cycle, Synchronization
National Category
Medical and Health Sciences Biological Sciences
URN: urn:nbn:se:uu:diva-137831DOI: 10.1016/j.exppara.2009.09.004ISI: 000273884300003OAI: oai:DiVA.org:uu-137831DiVA: diva2:379244
Available from: 2010-12-17 Created: 2010-12-16 Last updated: 2010-12-17Bibliographically approved

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