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Multilocus sequence typing of urogenital Chlamydia trachomatis from patients with different degrees of clinical symptoms
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology. (Herrmann)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology. (Herrmann)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Bacteriology. (Herrmann)
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2011 (English)In: Sexually Transmitted Diseases, ISSN 0148-5717, E-ISSN 1537-4521, Vol. 38, no 6, 490-494 p.Article in journal (Refereed) Published
Abstract [en]

Background: In the past, contradictory results have been obtained linking Chlamydia trachomatis serovars (ompA gene) to different clinical courses of infection.

Methods: A high resolution multilocus sequence typing (MLST) system was used to genotype 6 genetic regions, including ompA, in 70 Dutch urogenital C. trachomatis strains from patients with different degrees of defined clinical symptoms (asymptomatic, symptomatic, and lower abdominal pain), to determine if MLST genotypes correlated with clinical manifestations of infection.

Results and conclusions: We identified 46 MLST types, with only a small overlap to Swedish MLST types. This study found no correlation between MLST profiles and symptomatology. To understand the clinical course of infection, future studies should not only consider bacterial factors but also look on the immunogenetics of the host.

Place, publisher, year, edition, pages
2011. Vol. 38, no 6, 490-494 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-138699DOI: 10.1097/OLQ.0b013e31820b8be0ISI: 000290561200005OAI: oai:DiVA.org:uu-138699DiVA: diva2:379532
Available from: 2010-12-17 Created: 2010-12-17 Last updated: 2017-12-11Bibliographically approved
In thesis
1. High Resolution Genotyping of Chlamydia trachomatis
Open this publication in new window or tab >>High Resolution Genotyping of Chlamydia trachomatis
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Chlamydia trachomatis is an obligate intracellular bacterium of major human health concern, causing urogential chlamydia infections, lymphogranuloma venereum (LGV) and trachoma. Chlamydia is one of the most common sexually transmitted infections worldwide and can cause infertility.

In the first four papers described herein we used a high resolution multilocus sequence typing (MLST) system to investigate the epidemiology of C. trachomatis, and showed that MLST is superior to conventional ompA genotyping with respect to resolution. In the fifth paper we simplified the methodology by developing and validating a multilocus typing (MLT) DNA microarray based on the MLST system.

In more detail, MLST analysis of consecutive specimens from 2006 in Örebro County in Sweden, and comparison to specimens from 1999-2000, showed that the new variant C. trachomatis (nvCT) is monoclonal and likely has appeared in recent years.

MLST analysis of LGV specimens from men who have sex with men (MSM) showed that the increase of LGV in Europe in the last decade indeed was a clonal outbreak, contrary to the USA where LGV might have been present all along.

In the third paper, clinical symptoms could not be correlated with the MLST genotypes, suggesting, together with the combined results of all previous studies, that bacterial factors, if important, need to be understood in the context of host factors.

MLST analysis of specimens from a high incidence C. trachomatis area in North Norway revealed interesting epidemiological details concerning unusual genetic variants, the nvCT and MSM, but found no significant difference in genetic diversity compared to two other geographic areas in Norway.

Lastly, we developed a MLT array that provides high resolution while being rapid and cost-effective, which makes it an interesting alternative for C. trachomatis genotyping.

In conclusion, the MLST system and the MLT array have proven to be useful tools and should now be applied in further investigations to improve our understanding of C. trachomatis epidemiology.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2011. 48 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 690
Keyword
Chlamydia trachomatis: multilocus sequence typing: MLST: genotyping: lymphogranuloma venereum: new variant C. tracomatis: nvCT: multilocus typing DNA microarray: MLT array
National Category
Microbiology in the medical area
Research subject
Medical Science
Identifiers
urn:nbn:se:uu:diva-156751 (URN)978-91-554-8121-6 (ISBN)
Public defence
2011-09-29, Hörsalen, Klinisk mikrobiologi, Akademiska sjukhuset, Dag Hammarskjöldsväg 17, Uppsala, 09:15 (English)
Opponent
Supervisors
Available from: 2011-08-30 Created: 2011-08-09 Last updated: 2011-09-08

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Klint, MarkusHerrmann, Björn

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