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Application of skinned singel muscle fibres to determine myofilament function in ageing and disease
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience.
2007 (English)In: Journal of Musculoskeletal and Neuronal Interactions - JMNI, ISSN 1108-7161, Vol. 7, no 1, 56-61 p.Article in journal (Refereed) Published
Abstract [en]

The chemically skinned fibre is a suitable preparation to determine whether alterations in myofilament function contributeto muscle dysfunction during ageing and disorders such as chronic obstructive pulmonary disease (COPD). In this preparationthe sarcolemma is chemically permeabilized and the myofilament lattice kept intact, functioning under controlled near-phys-iological conditions. As force generating capacity is an important determinant of muscle function and is related to fibre cross-sectional area (FCSA), we compared several methods employed by researchers to determine FCSA. Specific tension, forcedivided by FCSA, has a co-efficient of variation of 27%, 37%, or 30% when the FCSA was measured from the width and depthassuming an elliptical circumference, the width assuming a circular circumference, and the width while the fibre was suspend-ed in the air, respectively. The last method showed the closest relation with the FCSA in histological sections. The velocity ofmaximal unloaded shortening (V0) varied with fibre type, with fibres expressing the ‚/slow (type I) myosin heavy chain(MyHC) isoform being the slowest and fibres expressing the IIb MyHC isoform the fastest. While muscle weakness experi-enced after surgery could not be explained by changes in specific tension or FCSA of individual fibres, the preparationrevealed significant changes in myofilament function during ageing and COPD.

Place, publisher, year, edition, pages
2007. Vol. 7, no 1, 56-61 p.
Keyword [en]
Skinned Singel Fibres, Specific Tension, Shortening Velocity, Ageing, COPD
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-10275OAI: oai:DiVA.org:uu-10275DiVA: diva2:38043
Available from: 2007-03-12 Created: 2007-03-12 Last updated: 2010-05-03Bibliographically approved

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Larsson, L
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