Sensitive protein detection via triple-binder proximity ligation assays
2007 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 4, no 2, 135-137 p.Article in journal (Refereed) Published
The detection of weakly expressed proteins and protein complexes in biological samples represents a fundamental challenge. We have developed a new proximity-ligation strategy named 3PLA that uses three recognition events for the highly specific and sensitive detection of as little as a hundred molecules of the vascular endothelial growth factor (VEGF), the biomarkers troponin I, and prostate-specific antigen (PSA) alone or in complex with an inhibitor--demonstrating the versatility of 3PLA.
Place, publisher, year, edition, pages
2007. Vol. 4, no 2, 135-137 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-10282DOI: 10.1038/nmeth974ISI: 000243998100014PubMedID: 17179939OAI: oai:DiVA.org:uu-10282DiVA: diva2:38050