uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
The WASP-binding protein WIRE has a role in the regulation of the actin filament system downstream of the platelet-derived growth factor receptor
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm, Ludwig Institute for Cancer Research.
2002 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 279, no 1, 21-33 p.Article in journal (Refereed) Published
Abstract [en]

Activation of growth factor receptors, such as platelet-derived growth factor (PDGF) receptors, has a major impact on the motile behavior of vertebrate cells. The WASP family of proteins has been recognized as important regulators of actin polymerization via the activation of the Arp2/3 complex. The activity of the WASP proteins has, in turn, been shown to be governed by a number of associated proteins, including the WASP interacting protein (WIP). This report presents a novel WIP-like protein, WIRE (for WIP-related). WIRE was shown to bind to the WH1 domain of WASP and N-WASP. WIRE was localized to actin filaments in transiently transfected PAE/PDGFRbeta cells, and in cells simultaneously expressing WIRE and WASP, WIRE relocalized WASP to actin filaments, a relocalization that required direct interaction between the two proteins. In addition, WIRE was able to bind the PDGF receptor substrate Nckbeta. PDGF treatment of cells ectopically expressing WIRE resulted in formation of peripheral protrusions composed of filopodia and lamellipodia-like structures. In cells expressing both WIRE and WASP, PDGF treatment induced a translocation of WASP to the cell margin, an effect that required the presence of WIRE. Taken together, the data presented indicate that WIRE has a role in the WASP-mediated organization of the actin cytoskeleton and that WIRE is a potential link between the activated PDGF receptor and the actin polymerization machinery.

Place, publisher, year, edition, pages
2002. Vol. 279, no 1, 21-33 p.
Keyword [en]
Amino Acid Sequence, Animals, COS Cells, Carrier Proteins/genetics/*physiology, Cells; Cultured, Contractile Proteins, Endothelium; Vascular/metabolism/ultrastructure, Focal Adhesions/metabolism, Humans, Microfilament Proteins/metabolism, Microfilaments/metabolism/*ultrastructure, Molecular Sequence Data, Nerve Tissue Proteins/metabolism, Profilins, Protein Transport, Proteins/*metabolism, RNA; Messenger/biosynthesis, Receptors; Platelet-Derived Growth Factor/*metabolism, Sequence Homology; Amino Acid, Signal Transduction, Swine, Wiskott-Aldrich Syndrome Protein, Wiskott-Aldrich Syndrome Protein Family, Wiskott-Aldrich Syndrome Protein; Neuronal
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-10339DOI: 10.1006/excr.2002.5576PubMedID: 12213210OAI: oai:DiVA.org:uu-10339DiVA: diva2:38107
Available from: 2007-03-16 Created: 2007-03-16 Last updated: 2013-10-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=12213210&dopt=Citation

Search in DiVA

By author/editor
Aspenström, Pontus
By organisation
Ludwig Institute for Cancer Research
In the same journal
Experimental Cell Research
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 158 hits
ReferencesLink to record
Permanent link

Direct link