RNAs actively cycle on the Sm-like protein Hfq
2010 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 24, no 23, 2621-2626 p.Article in journal (Refereed) Published
Hfq, a protein required for small RNA (sRNA)-mediated regulation in bacteria, binds RNA with low-nanomolar K-d values and long half-lives of complexes (>100 min). This cannot be reconciled with the 1-2-min response time of regulation in vivo. We show that RNAs displace each other on Hfq on a short time scale by RNA concentration-driven (active) cycling. Already at submicromolar concentrations of competitor RNA, half-lives of RNA-Hfq complexes are approximate to 1 min. We propose that competitor RNA associates transiently with RNA-Hfq complexes, RNAs exchange binding sites, and one of the RNAs eventually dissociates. This solves the "strong binding-high turn-over" paradox and permits efficient use of the Hfq pool.
Place, publisher, year, edition, pages
2010. Vol. 24, no 23, 2621-2626 p.
Hfq, sRNA, antisense RNA, post-transcriptional regulation, RNA chaperone, Lsm protein
IdentifiersURN: urn:nbn:se:uu:diva-139405DOI: 10.1101/gad.591310ISI: 000284835800006PubMedID: 21123649OAI: oai:DiVA.org:uu-139405DiVA: diva2:381289