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RNAs actively cycle on the Sm-like protein Hfq
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.
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2010 (English)In: Genes & Development, ISSN 0890-9369, E-ISSN 1549-5477, Vol. 24, no 23, 2621-2626 p.Article in journal (Refereed) Published
Abstract [en]

Hfq, a protein required for small RNA (sRNA)-mediated regulation in bacteria, binds RNA with low-nanomolar K-d values and long half-lives of complexes (>100 min). This cannot be reconciled with the 1-2-min response time of regulation in vivo. We show that RNAs displace each other on Hfq on a short time scale by RNA concentration-driven (active) cycling. Already at submicromolar concentrations of competitor RNA, half-lives of RNA-Hfq complexes are approximate to 1 min. We propose that competitor RNA associates transiently with RNA-Hfq complexes, RNAs exchange binding sites, and one of the RNAs eventually dissociates. This solves the "strong binding-high turn-over" paradox and permits efficient use of the Hfq pool.

Place, publisher, year, edition, pages
2010. Vol. 24, no 23, 2621-2626 p.
Keyword [en]
Hfq, sRNA, antisense RNA, post-transcriptional regulation, RNA chaperone, Lsm protein
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-139405DOI: 10.1101/gad.591310ISI: 000284835800006PubMedID: 21123649OAI: oai:DiVA.org:uu-139405DiVA: diva2:381289
Available from: 2010-12-27 Created: 2010-12-23 Last updated: 2017-12-11Bibliographically approved

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Elf, JohanWagner, Gerhart E. H.

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