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Syntaxin clusters assemble reversibly at sites of secretory granules in live cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology. (Barg)
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2010 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 107, no 48, 20804-20809 p.Article in journal (Refereed) Published
Abstract [en]

Syntaxin resides in the plasma membrane, where it helps to catalyze membrane fusion during exocytosis. The protein also forms clusters in cell-free and granule-free plasma-membrane sheets. We imaged the interaction between syntaxin and single secretory granules by two-color total internal reflection microscopy in PC12 cells. Syntaxin-GFP assembled in clusters at sites where single granules had docked at the plasma membrane. Clusters were intermittently present at granule sites, as syntaxin molecules assembled and disassembled in a coordinated fashion. Recruitment to granules required the N-terminal domain of syntaxin, but not the entry of syntaxin into SNARE complexes. Clusters facilitated exocytosis and disassembled once exocytosis was complete. Syntaxin cluster formation defines an intermediate step in exocytosis.

Place, publisher, year, edition, pages
2010. Vol. 107, no 48, 20804-20809 p.
Keyword [en]
molecular docking, dynamic instability, nanodomains, total internal reflection fluorescence
National Category
Medical and Health Sciences Neurosciences Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-139381DOI: 10.1073/pnas.1014823107ISI: 000284762400042PubMedID: 21076041OAI: oai:DiVA.org:uu-139381DiVA: diva2:381325
Available from: 2010-12-27 Created: 2010-12-23 Last updated: 2017-12-11Bibliographically approved

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