Occurrence of rheumatoid arthritis-associated autoantibodies in Sudanese patients with Leishmania donovani infection
(English)Manuscript (preprint) (Other academic)
Our aim with this investigation was to evaluate the occurrence of anti-cyclic citrullinated peptide antibodies (anti-CCP), rheumatoid factor (RF) and circulating immune complexes (IC) in Sudanese patients infected with Leishmania donovani parasite.
Serum samples were collected from Leishmania infected patients and healthy Sudanese controls. Sudanese anti-CCP positive RA patients were included as positive controls. Data from all analyses were also compared with Swedish healthy control cohorts. Levels of circulating IC and anti-CCP were measured using ELISA and RF using nephelometry. A control plate with cyclic control peptides containing arginine instead of citrulline was used to evaluate citrulline specific reactivity.
We demonstrate that sera from Leishmania infected patients are often RF positive, have elevated IC levels and that a substantial number exhibit antibody reactivity towards CCP. However, contrary to what was evident the in Sudanese RA sera, the CCP reactivity was not restricted to citrulline but reacted equally well with the arginine control peptide.
Our findings stress the importance to interpret a positive CCP test carefully when evaluated in non-rheumatic conditions.
Infectious Medicine Immunology in the medical area
Research subject Clinical Immunology
IdentifiersURN: urn:nbn:se:uu:diva-139528OAI: oai:DiVA.org:uu-139528DiVA: diva2:381559