A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis
2002 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 13, no 10, 3683-3695 p.Article in journal (Refereed) Published
Synthesis of the ribosomal subunits from pre-rRNA requires a large number of trans-acting proteins and small nucleolar ribonucleoprotein particles to execute base modifications, RNA cleavages, and structural rearrangements. We have characterized a novel protein, RNA-binding domain-1 (RBD-1), that is involved in ribosome biogenesis. This protein contains six consensus RNA-binding domains and is conserved as to sequence, domain organization, and cellular location from yeast to human. RBD-1 is essential in Caenorhabditis elegans. In the dipteran Chironomus tentans, RBD-1 (Ct-RBD-1) binds pre-rRNA in vitro and anti-Ct-RBD-1 antibodies repress pre-rRNA processing in vivo. Ct-RBD-1 is mainly located in the nucleolus in an RNA polymerase I transcription-dependent manner, but it is also present in discrete foci in the interchromatin and in the cytoplasm. In cytoplasmic extracts, 20-30% of Ct-RBD-1 is associated with ribosomes and, preferentially, with the 40S ribosomal subunit. Our data suggest that RBD-1 plays a role in structurally coordinating pre-rRNA during ribosome biogenesis and that this function is conserved in all eukaryotes.
Place, publisher, year, edition, pages
2002. Vol. 13, no 10, 3683-3695 p.
Animals, Caenorhabditis elegans/genetics/metabolism, Caenorhabditis elegans Proteins/genetics/*metabolism, Cell Nucleolus/metabolism, Chironomidae/anatomy & histology/genetics/*metabolism, Consensus Sequence, Fungal Proteins/genetics/metabolism, Hela Cells, Humans, Insect Proteins/genetics/metabolism, Mitosis/physiology, Nuclear Proteins/genetics/*metabolism, Protein Structure; Tertiary, RNA Interference, RNA Polymerase I/metabolism, RNA Processing; Post-Transcriptional, RNA; Ribosomal/metabolism, RNA-Binding Proteins/genetics/*metabolism, Ribonucleoproteins/metabolism, Ribosomes/*metabolism, Salivary Glands/cytology/metabolism, Transcription; Genetic
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-10405DOI: 10.1091/mbc.E02-03-0138PubMedID: 12388766OAI: oai:DiVA.org:uu-10405DiVA: diva2:38173