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Dab2 links CIN85 with clathrin-mediated receptor internalization.
Ludwiginstitutet för Cancerforskning.
Ludwiginstitutet för Cancerforskning.
2003 (English)In: FEBS Lett, ISSN 0014-5793, Vol. 554, no 1-2, 81-7 p.Article in journal (Refereed) Published
Abstract [en]

CIN85 is a multidomain scaffold protein involved in downregulation of receptor tyrosine kinases. Here we show that disabled-2 (Dab2), an endocytic adaptor molecule implicated in clathrin-coat assembly, associates with CIN85 in mammalian cells. All three SH3 domains of CIN85 were able to bind to the PKPAPR peptide in the carboxyl-terminal part of Dab2, possibly enabling CIN85 to simultaneously interact with multiple Dab2 molecules. CIN85 association with Dab2 is essential for its recruitment to clathrin coat and appears to be modulated by growth factor stimulation. Dab2 and clathrin dissociated from CIN85 following growth factor treatment, enabling other molecules, such as Cbl, to bind to CIN85. Taken together, our data indicate a dynamic interplay between CIN85 and its effectors during endocytosis of receptor tyrosine kinases.

Place, publisher, year, edition, pages
2003. Vol. 554, no 1-2, 81-7 p.
Keyword [en]
Adaptor Proteins; Signal Transducing, Adaptor Proteins; Vesicular Transport/*metabolism/physiology, Amino Acid Sequence, Animals, Binding Sites, Cell Line, Clathrin-Coated Vesicles, Conserved Sequence, Endocytosis, Genes; Tumor Suppressor, Growth Substances/pharmacology, Humans, Mice, Protein Binding, Receptor Protein-Tyrosine Kinases/*metabolism, Transfection, src Homology Domains
URN: urn:nbn:se:uu:diva-10678PubMedID: 14596919OAI: oai:DiVA.org:uu-10678DiVA: diva2:38446
Available from: 2007-04-18 Created: 2007-04-18 Last updated: 2011-01-13

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ReferencesLink to record
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