Visualizing a set of olfactory sensory neurons responding to a bile salt
2011 (English)In: Journal of Experimental Biology, ISSN 0022-0949, Vol. 214, no 1, 80-87 p.Article in journal (Refereed) Published
In the present study, we exposed the olfactory epithelia of crucian carp, Carassius carassius, and brown trout, Salmo trutta, to dextran coupled with Alexa dyes together with odorants. Dye uptake was severely reduced after pre-exposure to nocodazole, an inhibitor of microtubule polymerization that impairs endocytosis, supporting the hypothesis that odour-activated olfactory receptor molecules undergo endocytosis. Application of the bile acid taurolithocholate, a potent and specific odorant for fish, resulted in the labelling of a sparse (less than 3%) cell population with the typical morphology of ciliated sensory neurons (CSNs) - long dendrites and cell somata deep in the sensory epithelium. The dye was distributed throughout the sensory neuron, also revealing axons and target glomeruli. Stained axons redistribute at the entrance of the olfactory bulb and terminate in two small target areas, a dorsal and a medial one. These results are consistent with the notion that taurolithocholate is detected specifically by a few ciliated sensory neurons. Application of the olfactory epithelium of brown trout to bile acid stained cells with the appearance of CSNs. Application of an alarm agonist, hypxanthine-3-N-oxide, to crucian carp olfactory organ caused staining of another set of sensory neurons. Furthermore, our results show that odour-induced uptake of a dye can serve to identify the subtype of olfactory sensory neurons responding to a particular odorant, and to pinpoint the target regions of these neurons in the olfactory bulb as a first step to elucidating the neuronal network responding to a particular odour.
Place, publisher, year, edition, pages
2011. Vol. 214, no 1, 80-87 p.
endocytosis, ligand specificity, projection pathways, network, ciliated sensory neurons
IdentifiersURN: urn:nbn:se:uu:diva-140946DOI: 10.1242/jeb.046607ISI: 000285090000017PubMedID: 21147971OAI: oai:DiVA.org:uu-140946DiVA: diva2:384802