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Recombinant spider silk as matrices for cell culture
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
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2010 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 31, no 36, 9575-9585 p.Article in journal (Refereed) Published
Abstract [en]

The recombinant miniature spider silk protein, 4RepCT, was used to fabricate film, foam, fiber and mesh matrices of different dimensionality, microstructure and nanotopography. These matrices were evaluated regarding their suitability for cell culturing. Human primary fibroblasts attached to and grew well on all matrix types, also in the absence of serum proteins or other animal-derived additives. The highest cell counts were obtained on matrices combining film and fiber/mesh. The cells showed an elongated shape that followed the structure of the matrices and exhibited prominent actin filaments. Moreover, the fibroblasts produced, secreted and deposited collagen type I onto the matrices. These results, together with findings of the matrices being mechanically robust, hold promise not only for in vitro cell culturing, but also for tissue engineering applications.

Place, publisher, year, edition, pages
2010. Vol. 31, no 36, 9575-9585 p.
Keyword [en]
Biomaterial, Collagen production, Cytocompatibility, Fibroblasts, Serum free, Tissue engineering
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-138973DOI: 10.1016/j.biomaterials.2010.08.061ISI: 000285120800022PubMedID: 20875917OAI: oai:DiVA.org:uu-138973DiVA: diva2:385093
Available from: 2011-01-11 Created: 2010-12-21 Last updated: 2011-02-24Bibliographically approved

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