uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Expansion and Activation Kinetics of Immune Cells during Early Phase of GVHD in Mouse Model Based on Chemotherapy Conditioning
Show others and affiliations
2010 (English)In: Clinical & Developmental Immunology, ISSN 1740-2522, E-ISSN 1740-2530, Vol. 2010, 142943- p.Article in journal (Refereed) Published
Abstract [en]

In the present paper, we have investigated early pathophysiological events in graft-versus-host disease (GVHD), a major complication to hematopoietic stem cell transplantation (HSCT). BLLB/c female mice conditioned with busulfan/cyclophosphamide (Bu-Cy) were transplanted with allogeneic male C57BL/6. Control group consisted of syngeneic transplanted Balb/c mice. In allogeneic settings, significant expansion and maturation of donor dendritic cells (DCs) were observed at day +3, while donor T-cells CD8+ were increased at day +5 (230%) compared to syngeneic HSCT. Highest levels of inflammatory cytokines IL-2, IFN-gamma, and TNF-alfa at day +5 matched T-cell activation. Concomitantly naïve T-cells gain effecr-memory phenotype and migrated from spleen to peripheral lymphoid organs. Thus, in the very early phase of GHVD following Bu-Cy conditioning donor, DCs play an important role in the activation of donor T cells. Subsequently, donor naïve T-cells gain effector-memory phenotype and initiate GVHD.

Place, publisher, year, edition, pages
2010. Vol. 2010, 142943- p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-141228DOI: 10.1155/2010/142943PubMedID: 21197273OAI: oai:DiVA.org:uu-141228DiVA: diva2:385266
Available from: 2011-01-11 Created: 2011-01-11 Last updated: 2011-01-26Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Medical Biochemistry and Microbiology
In the same journal
Clinical & Developmental Immunology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 278 hits
ReferencesLink to record
Permanent link

Direct link