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Blood protein-polymer adsorption: Implications for understanding complement-mediated hemoincompatibility
University of Pennsylvania.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Clinical Immunology. (Bo Nilsson)
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2011 (English)In: Journal of Biomedical Materials Research - Part A, ISSN 1549-3296, Vol. 97A, no 1, 74-84 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to create polymeric materials with known properties to study the preconditions for complement activation. Initially, 22 polymers were screened for complement activating capacity. Based on these results, six polymers (P1-P6) were characterized regarding physico-chemical parameters, for example, composition, surface area, pore size, and protein adsorption from human EDTA-plasma. P2, P4, and reference particles of polystyrene and polyvinyl chloride, were hydrophobic, bound low levels of protein and were poor complement activators. Their accessible surface was limited to protein adsorption in that they had pore diameters smaller than most plasma proteins. P1 and P3 were negatively charged and adsorbed IgG and C1q. A 10-fold difference in complement activation was attributed to the fact that P3 but not P1 bound high amounts of C1-inhibitor. The hydrophobic P5 and P6 were low complement activators. They selectively bound apolipoproteins AI and AIV (and vitronectin), which probably limited the binding of complement activators to the surface. We demonstrate the usefulness of the modus operandi to use a high-throughput procedure to synthesize a great number of novel substances, assay their physico-chemical properties with the aim to study the relationship between the initial protein coat on a surface and subsequent biological events. Data obtained from the six polymers characterized here, suggest that a complement-resistant surface should be hydrophobic, uncharged, and have a small available surface, accomplished by nanostructured topography. Additional attenuation of complement can be achieved by selective enrichment of inert proteins and inhibitors.

Place, publisher, year, edition, pages
2011. Vol. 97A, no 1, 74-84 p.
Keyword [en]
Polymers, Biomaterials, Complement, Plasma protein adsorption hemocompatibility
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-142008DOI: 10.1002/jbm.a.33030ISI: 000288305500010PubMedID: 21319295OAI: oai:DiVA.org:uu-142008DiVA: diva2:386746
Available from: 2011-01-13 Created: 2011-01-13 Last updated: 2011-04-04Bibliographically approved

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