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Protective effect of brain-derived neurotrophic factor against the ototoxicity of Pseudomonas aeruginosa exotoxin A
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences. (Ear-Nose-Throat)
2003 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, Vol. 123, no 1, 14-9 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: The protective effect of brain-derived neurotrophic factor (BDNF) against the ototoxicity resulting from exposure of Pseudomonas aeruginosa exotoxin A (PaExoA) to the middle ear was analyzed. The combined effect of BDNF and N(G)-nitro-L-arginine methyl ester (L-NAME) was also investigated. MATERIAL AND METHODS: Six groups of albino rats were instilled through the tympanic membrane into the round window niche with the following solutions: saline; PaExoA; BDNF; L-NAME; PaExoA + BDNF; and PaExoA + BDNF + L-NAME. Frequency-specific (2-31.5 kHz) auditory brainstem responses were used to obtain the hearing thresholds before and 2, 5 and 15 days after instillation. RESULTS: PaExoA penetrated from the middle ear into the cochlea, causing initially mixed hearing loss, followed by persistent sensorineural hearing loss. This impairment was blocked by BDNF at 6, 8 and 10 kHz on Day 2 and at 8 kHz on Day 5. L-NAME given in combination with BDNF did not show any additional protective effect. There were no significant differences in the thickness of the round window membrane between control ears and those in each instillation group. CONCLUSION: Our results suggest that BDNF may protect against cochlear damage caused by PaExoA in the middle turns of the ear.

Place, publisher, year, edition, pages
2003. Vol. 123, no 1, 14-9 p.
Keyword [en]
ADP Ribose Transferases/*toxicity, Animals, Audiometry; Pure-Tone, Auditory Fatigue/drug effects, Auditory Threshold/drug effects, Bacterial Toxins/*toxicity, Brain-Derived Neurotrophic Factor/*pharmacology, Cochlear Nerve/drug effects, Ear; Middle/*drug effects, Evoked Potentials; Auditory; Brain Stem/*drug effects, Exotoxins/*toxicity, Hearing Loss; Sensorineural/*physiopathology, Male, NG-Nitroarginine Methyl Ester/pharmacology, Neuroprotective Agents/*pharmacology, Pitch Perception/*drug effects, Round Window/drug effects, Virulence Factors/*toxicity
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-10927PubMedID: 12625567OAI: oai:DiVA.org:uu-10927DiVA: diva2:38695
Available from: 2007-05-04 Created: 2007-05-04 Last updated: 2010-03-03Bibliographically approved

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Anniko, Matti
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