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p53 targets identified by protein expression profiling
Department of Oncology–Pathology, Cancer Center Karolinska, Karolinska Institutet, SE-171 76 Stockholm, Sweden.
Department of Surgery, University of Schleswig-Holstein, Campus Lu¨ beck, Ratzeburger Allee 160, D-23538 Lu¨ beck, Germany.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
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2007 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 104, no 13, 5401-5406 p.Article in journal (Refereed) Published
Abstract [en]

p53 triggers cell cycle arrest and apoptosis through transcriptional regulation of specific target genes. We have investigated the effect of p53 activation on the proteome using 2D gel electrophoresis analysis of mitomycin C-treated HCT116 colon carcinoma cells carrying wild-type p53. Approximately 5,800 protein spots were separated in overlapping narrow-pH-range gel strips, and 115 protein spots showed significant expression changes upon p53 activation. The identity of 55 protein spots was obtained by mass spectrometry. The majority of the identified proteins have no previous connection to p53. The proteins fall into different functional categories, such as mRNA processing, translation, redox regulation, and apoptosis, consistent with the idea that p53 regulates multiple cellular pathways. p53-dependent regulation of five of the up-regulated proteins, eIF5A, hnRNP C1/C2, hnRNP K, lamin A/C, and Nm23-H1, and two of the down-regulated proteins, Prx II and TrpRS, was examined in further detail. Analysis of mRNA expression levels demonstrated both transcription-dependent and transcription-independent regulation among the identified targets. Thus, this study reveals protein targets of p53 and highlights the role of transcription-independent effects for the p53-induced biological response.

Place, publisher, year, edition, pages
2007. Vol. 104, no 13, 5401-5406 p.
Keyword [en]
Proteomics, transcription factor, cancer
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-11020DOI: 10.1073/pnas.0700794104ISI: 000245331700028PubMedID: 17372198OAI: oai:DiVA.org:uu-11020DiVA: diva2:38788
Available from: 2007-05-11 Created: 2007-05-11 Last updated: 2017-12-11Bibliographically approved

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