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Lack of specificity of the molecular diagnosis method for identification of Aphanomyces astaci
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Physiology and Developmental Biology, Comparative Physiology.
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2007 (English)In: BFPP-CONNAISSANCE ET GESTION DU PATRIMOINE AQUATIQUE, ISSN 1297-6318, no 385, 17-24 p.Article in journal (Refereed) Published
Abstract [en]

A recent PCR-test developed for identification of Aphanomyces astaci, the organism responsible for crayfish plague, provided false positives for Aphanomyces frigidophilus, Aphanomyces repetans, and some Saprolegnia spp. Real-time PCR showed that with the designed primers, A. astaci and A. frigidophilus cannot be distinguished. The results of this study show that this particular crayfish plague PCR-test ought to be improved and that molecular-based techniques need to be contrasted to histological evidences and disease history.

Abstract [fr]

Un test PCR récemment développé pour identifier Aphanomyces astaci, organisme responsable de la peste de l'écrevisse, a donné des faux positifs pour Aphanomyces frigidophilus, Aphanomyces repetans, et pour quelques Saprolegnia spp. La PCR en temps réel montre, qu'avec les "primers" redessinés, A. astaci et A. frigidophilus ne peuvent pas être distingués. Les résultats de cette étude montrent que ce test PCR, conçu pour identifier la peste de l'écrevisse, doit être amélioré et que les techniques moléculaires doivent être confortées par des évidences histologiques et en fonction de l´histoire de la maladie.

Place, publisher, year, edition, pages
2007. no 385, 17-24 p.
Keyword [en]
Oomycetes, crayfish plague, ITS, crayfish, Conservation, invasive species
Keyword [fr]
Oomycètes, peste de l'écrevisse, ITS, écrevisse, conservation, espèces invasives
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-11242DOI: 10.1051/kmae:2007002ISI: 000249580600002OAI: oai:DiVA.org:uu-11242DiVA: diva2:39010
Available from: 2008-05-15 Created: 2008-05-15 Last updated: 2016-05-15

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Cerenius, LageSöderhäll, Kenneth

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