Isoflurane-induced acidosis depresses basal and PGE(2)-stimulated duodenal bicarbonate secretion in mice
2007 (English)In: American Journal of Physiology - Gastrointestinal and Liver Physiology, ISSN 0193-1857, E-ISSN 1522-1547, Vol. 292, no 3, G899-G904 p.Article in journal (Refereed) Published
When running in vivo experiments, it is imperative to keep arterial blood pressure and acid-base parameters within the normal physiological range. The aim of this investigation was to explore the consequences of anesthesia-induced acidosis on basal and PGE2-stimulated duodenal bicarbonate secretion. Mice (strain C57bl/6J) were kept anesthetized by a spontaneous inhalation of isoflurane. Mean arterial blood pressure (MAP), arterial acid-base balance, and duodenal mucosal bicarbonate secretion (DMBS) were studied. Two intra-arterial fluid support strategies were used: a standard Ringer solution and an isotonic Na2CO3 solution. Duodenal single perfusion was used, and DMBS was assessed by back titration of the effluent. PGE2 was used to stimulate DMBS. In Ringer solution-infused mice, isoflurane-induced acidosis became worse with time. The blood pH was 7.15–7.21 and the base excess was about –8 mM at the end of experiments. The continuous infusion of Na2CO3 solution completely compensated for the acidosis. The blood pH was 7.36–7.37 and base excess was about 1 mM at the end of the experiment. Basal and PGE2-stimulated DMBS were markedly greater in animals treated with Na2CO3 solution than in those treated with Ringer solution. MAP was slightly higher after Na2CO3 solution infusion than after Ringer solution infusion. We concluded that isoflurane-induced acidosis markedly depresses basal and PGE2-stimulated DMBS as well as the responsiveness to PGE2, effects prevented by a continuous infusion of Na2CO3. When performing in vivo experiments in isoflurane-anesthetized mice, it is recommended to supplement with a Na2CO3 infusion to maintain a normal acid-base balance.
Place, publisher, year, edition, pages
2007. Vol. 292, no 3, G899-G904 p.
Acid-Base Equilibrium/drug effects, Acidosis/blood/chemically induced/*metabolism, Animals, Bicarbonates/*metabolism, Blood Pressure/drug effects, Carbon Dioxide/blood, Carbonates/blood/pharmacology, Dinoprostone/*pharmacology, Duodenum/*drug effects/secretion, Hydrogen-Ion Concentration/drug effects, Intestinal Mucosa/drug effects/secretion, Isoflurane, Mice, Mice; Inbred C57BL, Partial Pressure, Perfusion
Medical and Health Sciences Pharmaceutical Sciences
IdentifiersURN: urn:nbn:se:uu:diva-11249DOI: 10.1152/ajpgi.00398.2006ISI: 000244787200022PubMedID: 17158257OAI: oai:DiVA.org:uu-11249DiVA: diva2:39017