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Towards biosensing of arteriosclerotic nanoplaque formation using femtosecond spectroscopy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
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2007 (English)In: Archives of Biochemistry and Biophysics, ISSN 0003-9861, E-ISSN 1096-0384, Vol. 460, no 1, 92-99 p.Article in journal (Refereed) Published
Abstract [en]

The ultrafast dynamics of proteoheparan sulfate (HS-PG) in Krebs blood substitute solution was measured using femtosecond transient absorption spectroscopy after UV excitation. Interacting with blood lipoproteins and Ca2+ ions, the proteoglycan HS-PG is the key component of the so-called nanoplaque, the earliest stage in atherogenesis. Since tryptophan (Trp) residues are the main optically active parts of HS-PG, analogous measurements were performed on bare Trp in Krebs solution. The comparison reveals distinct differences to main characteristics of the HS-PG broadband absorption spectra. Analyzing the Trp spectra, we show that the results from transient absorption spectroscopy resemble the time constants of the chromophore ultrafast solvation dynamics that have been found by another group using fluorescence up-conversion techniques. Yet, the broadband transient absorption provides more details about the molecular dynamics, including stimulated emission, excited state absorption and resonant energy transfer. Furthermore, the absorption long time dynamics upon adding Ca2+ to the HS-PG probe were investigated by transient absorption spectroscopy and by surface force and ellipsometry investigations. Notably, a Ca2+-induced conformational change responsible for arteriosclerotic nanoplaque formation was detected. Slight differences, which are only visible as broad spectral features in the sub-picosecond time scale, provide a first insight into the molecular formation of nanoplaques in blood vessels, which may yield a better understanding of the genesis of arteriosclerosis.

Place, publisher, year, edition, pages
2007. Vol. 460, no 1, 92-99 p.
Keyword [en]
Proteoheparan sulfate, Arteriosclerotic nanoplaque formation, Proteoglycan conformation and solvation, Femtosecond transient absorption spectroscopy, Surface force measurements
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-11471DOI: 10.1016/j.abb.2007.01.018ISI: 000245571900012PubMedID: 17324375OAI: oai:DiVA.org:uu-11471DiVA: diva2:39240
Available from: 2007-09-17 Created: 2007-09-17 Last updated: 2017-12-11Bibliographically approved

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Malmsten, Martin

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