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Albumin-bound lipids induce free cytoplasmic calcium oscillations in human osteoblast-like cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
2007 (English)In: Cell Biochemistry and Function, ISSN 0263-6484, E-ISSN 1099-0844, Vol. 25, no 3, 245-249 p.Article in journal (Refereed) Published
Abstract [en]

[Ca2+](i) oscillations were found in human osteoblast-like cells (hOB cells) exposed to high-lipid bovine serum albumin (BSA), but not when exposed to low-lipid BSA. These [Ca2+](i) oscillations were inhibited by heptanol and suramin, which implies that gap junctions and purinergic signalling may be important for these [Ca2+](i) oscillations. The high-lipid BSA preparation that was used contains arachidonic acid. [Ca2+](i) oscillations could be induced by low lipid albumin with arachidonic acid added. The albumin-bound lipids were also important for osteoblast growth since DNA synthesis and the total cell protein content was higher in hOB cells exposed to high-lipid BSA. The effect of arachidonic acid on hOB cell proliferation was bone-donor dependent; both stimulatory and inhibitory effects were observed. The physiological importance of albumin-bound lipids is unclear; given that albumin has only minimal contact with osteoblasts under normal conditions. Only when bone capillaries are disrupted, e.g. during a fracture, would significant amounts of albumin reach osteoblasts. Albumin-bound lipids could therefore contribute to stimulation of osteoblast proliferation during fracture healing.

Place, publisher, year, edition, pages
2007. Vol. 25, no 3, 245-249 p.
Keyword [en]
osteoblast, [Ca2+](i) oscillations, lipids, albumin
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-144468DOI: 10.1002/cbf.1316ISI: 000246778000001PubMedID: 16397911OAI: oai:DiVA.org:uu-144468DiVA: diva2:393459
Available from: 2011-01-31 Created: 2011-01-31 Last updated: 2011-01-31Bibliographically approved

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