Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis
2007 (English)In: Chemistry Letters, ISSN 0366-7022, E-ISSN 1348-0715, Vol. 36, no 3, 396-397 p.Article in journal (Refereed) Published
A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (it = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.
Place, publisher, year, edition, pages
2007. Vol. 36, no 3, 396-397 p.
IdentifiersURN: urn:nbn:se:uu:diva-144906ISI: 000246058000023OAI: oai:DiVA.org:uu-144906DiVA: diva2:394675