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Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2007 (English)In: Chemistry Letters, ISSN 0366-7022, E-ISSN 1348-0715, Vol. 36, no 3, 396-397 p.Article in journal (Refereed) Published
Abstract [en]

A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (it = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.

Place, publisher, year, edition, pages
2007. Vol. 36, no 3, 396-397 p.
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Chemical Sciences
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URN: urn:nbn:se:uu:diva-144906ISI: 000246058000023OAI: oai:DiVA.org:uu-144906DiVA: diva2:394675
Available from: 2011-02-03 Created: 2011-02-03 Last updated: 2017-12-11Bibliographically approved

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