uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
A new quantitative real-time reverse transcriptase PCR assay and melting curve analysis for detection and genotyping of Ljungan virus strains
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Show others and affiliations
2007 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 141, no 1, 71-77 p.Article in journal (Refereed) Published
Abstract [en]

Ljungan virus (LV), a new member of the Picornaviridoe, recently isolated from vole species in both Sweden and the USA, is suspected to be pathogenic for humans as an actiological agent in myocarditis, diabetes, neurological disease and perinatal disease. This study describes for the first time an RT-PCR assay that can identify and quantify LV infection in various tissue sample types using primers and two different minor-groove-binder probes targeting the 5'-untranslated region of the LV genome. The assay, evaluated using control samples derived from various virus cultures and rodent tissues, allows precise quantification of viral load over six orders of magnitude (101 to 106 viral copies per assay) for all known strains of LV with high sensitivity and specificity. Furthermore, a melting curve analysis (MCA) was developed using two amplicon-specific hybridisation probes that allows rapid genotyping of different LV strains. These new methods provide useful tools to investigate the putative role of LV as a pathogen in both rodents and humans.

Place, publisher, year, edition, pages
2007. Vol. 141, no 1, 71-77 p.
Keyword [en]
Ljungan virus, viral zoonoses, aetiological diagnostic, real-time RT-PCR, minor-groove-binder probes, melting curve analysis
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-145490DOI: 10.1016/j.jviromet.2006.11.029ISI: 000245150100011PubMedID: 17196265OAI: oai:DiVA.org:uu-145490DiVA: diva2:396268
Available from: 2011-02-09 Created: 2011-02-09 Last updated: 2011-02-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Department of Medical Cell Biology
In the same journal
Journal of Virological Methods
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 172 hits
ReferencesLink to record
Permanent link

Direct link