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An investigation of peak-broadening effects arising when combining CE with MS.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
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2007 (English)In: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 28, no 18, 3207-3213 p.Article in journal (Refereed) Published
Abstract [en]

In this study, peak-broadening effects caused by nebulizing gas flow and lack of temperature control have been investigated for separation capillaries with three different inner diameters. The study was performed with serial UV/ESI-MS detection in an effort to distinguish between peak broadening arising in the separation and peak broadening arising in the ion source. The nebulizing gas was found to significantly affect both migration time and separation efficiency when using capillaries with 50 and 75 µm id. If the nebulizing gas is on during injection, the injection volume increases to such an extent that significant peak broadening is induced. Reducing the id to 25 µm minimizes the parabolic flow induced by the nebulizing gas. Results indicate that the nebulizing gas pressure can be optimized to minimize peak broadening in the ion source. A decrease in detection sensitivity, possibly related to the orthogonal design of the interface, was observed when the nebulizing gas pressure was increased. A tapered capillary tip was found to provide superior separation efficiency as well as sensitivity.

Place, publisher, year, edition, pages
2007. Vol. 28, no 18, 3207-3213 p.
Keyword [en]
CE, ESI, MS, peak broadening, UV
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-11867DOI: 10.1002/elps.200700158ISI: 000250039000018PubMedID: 17854122OAI: oai:DiVA.org:uu-11867DiVA: diva2:39636
Available from: 2007-10-30 Created: 2007-10-30 Last updated: 2011-03-25Bibliographically approved

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Axén, JakobSjöberg, Per J. R.
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