uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
A novel histology-directed strategy for MALDI-MS tissue profiling that improves throughput and cellular specificity in human breast cancer.
Vanderbilt University. (Analytical Chemistry, MSRC)
Show others and affiliations
2006 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 5, no 10, 1975-83 p.Article in journal (Refereed) Published
Abstract [en]

We describe a novel tissue profiling strategy that improves the cellular specificity and analysis throughput of protein profiles obtained by direct MALDI analysis. The new approach integrates the cellular specificity of histology, the accuracy and reproducibility of robotic liquid dispensing, and the speed and objectivity of automated spectra acquisition. Traditional methodologies for preparing and analyzing tissue samples rely heavily on manual procedures, which for various reasons discussed, restrict cellular specificity and sample throughput. Here, a robotic spotter deposits micron-sized droplets of matrix precisely onto foci of normal mammary epithelium, ductal carcinoma in situ, invasive mammary cancer, and peritumoral stroma selected by a pathologist from high resolution histological images of sectioned human breast cancer samples. The location of each matrix spot was then determined and uploaded into the instrument to facilitate automated profile acquisition by MALDI-TOF. In the example shown, the different lesions were clearly differentiated using mass profiling. Further, the workflow permits a visual projection of any information produced from the profile analyses directly on the histological image for a unique combination of proteomic and histological assessment of sample regions. The higher performance characteristics offered by the new workflow promises to be a significant advancement toward the next generation of tissue profiling studies.

Place, publisher, year, edition, pages
2006. Vol. 5, no 10, 1975-83 p.
URN: urn:nbn:se:uu:diva-145671DOI: 10.1074/mcp.M600119-MCP200PubMedID: 16849436OAI: oai:DiVA.org:uu-145671DiVA: diva2:396577
Available from: 2011-02-10 Created: 2011-02-10 Last updated: 2011-03-16

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Andersson, Malin
In the same journal
Molecular & Cellular Proteomics

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 125 hits
ReferencesLink to record
Permanent link

Direct link