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tmRNA-SmpB complex mimics native aminoacyl-tRNAs in the A site of stalled ribosomes.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. (ehrenberg)
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. (ehrenberg)
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2010 (English)In: Journal of Structural Biology, ISSN 1047-8477, E-ISSN 1095-8657, Vol. 169, no 3, 342-348 p.Article in journal (Refereed) Published
Abstract [en]

Bacterial ribosomes stalled on faulty, often truncated, mRNAs lacking stop codons are rescued by trans-translation. It relies on an RNA molecule (tmRNA) capable of replacing the faulty mRNA with its own open reading frame (ORF). Translation of tmRNA ORF results in the tagging of faulty protein for degradation and its release from the ribosome. We used single-particle cryo-electron microscopy to visualize tmRNA together with its helper protein SmpB on the 70S Escherichia coli ribosome in states subsequent to GTP hydrolysis on elongation factor Tu (EF-Tu). Three-dimensional reconstruction and heterogeneity analysis resulted in a 15 A resolution structure of the tmRNA-SmpB complex accommodated in the A site of the ribosome, which shows that SmpB mimics the anticodon- and D-stem of native tRNAs missing in the tRNA-like domain of tmRNA. We conclude that the tmRNA-SmpB complex accommodates in the ribosomal A site very much like an aminoacyl-tRNA during protein elongation.  

Place, publisher, year, edition, pages
2010. Vol. 169, no 3, 342-348 p.
Keyword [en]
Trans-translation, tmRNA, SmpB, cryo-electron microscopy, single-particle, heterogeneity analysis
Identifiers
URN: urn:nbn:se:uu:diva-145781DOI: 10.1016/j.jsb.2009.10.015ISI: 000275296100010PubMedID: 19883769OAI: oai:DiVA.org:uu-145781DiVA: diva2:396817
Available from: 2011-02-11 Created: 2011-02-11 Last updated: 2017-12-11Bibliographically approved

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