Development and validation of an automated solid-phase extraction and liquid chromatographic method for determination of lumefantrine in capillary blood on sampling paper
2007 (English)In: Journal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085, E-ISSN 1873-264X, Vol. 45, no 2, 282-287 p.Article in journal (Refereed) Published
A bioanalytical method for the determination of lumefantrine in 100 μl blood applied onto sampling paper, by solid-phase extraction and liquid chromatography, has been developed and validated. Whatman 31 ET Chr sampling paper was pre-treated with 0.75 M tartaric acid before sampling capillary blood to enable a high recovery of lumefantrine. Lumefantrine was extracted from the sampling paper, then further purified using solid-phase extraction and finally quantified with HPLC. The between-day variation was below 10% over the range 0.4-25 μM. The lower limit of quantification was 0.25 μM in 100 μl capillary blood. No decrease in lumefantrine concentration in dried blood spot is seen after 4 months storage at 22 °C. The method was also evaluated in field samples from patients in Tanzania after treatment with lumefantrine/artemether. Lumefantrine could be estimated accurately enough to assess bioavailability and treatment compliance on day 7 (i.e. 4 days after the last dose) after a standard regimen with the lumefantrine/artemether combination.
Place, publisher, year, edition, pages
2007. Vol. 45, no 2, 282-287 p.
Lumefantrine, Sampling paper, Dried blood spots, Capillary blood, Antimalarial drugs, Solid-phase extraction, Liquid chromatography
IdentifiersURN: urn:nbn:se:uu:diva-11919DOI: 10.1016/j.jpba.2007.07.015ISI: 000250888700014PubMedID: 17719735OAI: oai:DiVA.org:uu-11919DiVA: diva2:39688