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Synthetic de novo designed polypeptides for control of nanoparticle assembly and biosensing
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry.
2007 (English)In: Biochemical Society Transactions, ISSN 0300-5127, E-ISSN 1470-8752, Vol. 35, no 3, 532-534 p.Article in journal (Refereed) Published
Abstract [en]

This contribution describes how de novo designed synthetic helix–loop–helix polypeptides are utilized tocontrol the assembly of gold nanoparticles and as scaffolds for biosensing. The synthetic polypeptides aredesigned to fold into a four-helix bundle upon dimerization. When immobilized on gold nanoparticles,dimerization and folding occur between peptides on neighbouring particles as an effect of particleaggregation and the folded polypeptides are rigid enough to keep the particles separated at a distancecorresponding to the size of the four-helix bundle. Moreover, peptide dimerization offers a convenientroute to assemble nanoparticles into hybrid multilayers on planar substrates. The drastic change in theresonance conditions of the localized nanoparticle surface plasmon upon particle aggregation is shown tobe useful for optical detection of biomolecular interactions.

Place, publisher, year, edition, pages
2007. Vol. 35, no 3, 532-534 p.
Keyword [en]
biosensing, folding, four-helix bundle, gold nanoparticle, nanoparticle assembly, polypeptide scaffold
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-12014DOI: 10.1042/BST0350532ISI: 000247444900025PubMedID: 17511645OAI: oai:DiVA.org:uu-12014DiVA: diva2:39783
Available from: 2007-11-26 Created: 2007-11-26 Last updated: 2011-04-12Bibliographically approved

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