Immunolocalization of prestin in human Cochlea
2010 (English)In: Audiological Medicine, ISSN 1651-386X, E-ISSN 1651-3835, Vol. 8, no 2, 56-62 p.Article in journal (Refereed) Published
Prestin is an anion-transporter-related protein highly expressed in mammalian outer hair cells (OHCs). It is associated
with the OHC basolateral plasma membrane and responsible for cell body contraction, increasing cochlear sensitivity
and frequency resolution. Here, we analysed the expression and distribution of prestin in the human cochlea.
Immunohistochemistry including confocal microscopy and SEM were performed on EDTA-decalcifi ed human cochlea
removed during petro-clival meningioma surgery.
Prestin was found to be expressed solely in OHCs. No staining
was seen in IHCs. Prestin immunolabelling framed the OHCs along the sensory region of the human cochlea. Staining was
most prominent in the lateral cell membrane with less expression in the sub- and peri-nuclear part of the cell. Prestin immunostaining
was also detected in the OHC cytosol, presumably refl ecting intracellular molecular traffi cking.
study describes for the fi rst time the localization and distribution of prestin in the well preserved human organ of Corti.
Place, publisher, year, edition, pages
2010. Vol. 8, no 2, 56-62 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-146302DOI: 10.3109/16513860903320300OAI: oai:DiVA.org:uu-146302DiVA: diva2:397958