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Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
2007 (English)In: Nature Protocols, ISSN 1754-2189, Vol. 2, no 9, 2111-2119 p.Article in journal (Refereed) Published
Abstract [en]

Permeability coefficients across monolayers of the human colon carcinoma cell line Caco-2, cultured on permeable supports, are commonly used to predict the absorption of orally administered drugs and other xenobiotics. This protocol describes our method for the cultivation, characterization and determination of permeability coefficients of xenobiotics (which are, typically, drug-like compounds) in the Caco-2 model. A few modifications that have been introduced over the years are incorporated in the protocol. The method can be used to trace the permeability of a test compound in two directions, from the apical to the basolateral side or vice versa, and both passive and active transport processes can be studied. The permeability assay can be completed within one working day, provided that the Caco-2 monolayers have been cultured and differentiated on the permeable supports 3 weeks in advance.

Place, publisher, year, edition, pages
2007. Vol. 2, no 9, 2111-2119 p.
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-12029DOI: 10.1038/nprot.2007.303ISI: 000253139600007PubMedID: 17853866OAI: oai:DiVA.org:uu-12029DiVA: diva2:39798
Available from: 2007-11-19 Created: 2007-11-19 Last updated: 2018-01-12Bibliographically approved

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Hubatsch, InaArtursson, Per

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