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Universal method for synthesis of artificial gel antibodies by the imprinting approach combined with a unique electrophoresis technique for detection of minute structural differences of proteins, viruses, and cells (bacteria): II. Gel antibodies against virus (Semliki Forest Virus)
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Biochemistry and Organic Chemistry, Biochemistry.
2006 (English)In: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 29, no 18, 2810-2815 p.Article in journal (Refereed) Published
Abstract [en]

Artificial and highly selective antibodies (in the form of gel granules) against proteins can easily be synthesized by a simple, cost-effective imprinting technique [Liao, J.-L. et al., Chromatographia 1996, 42, 259-262]. Using the same method for synthesis of gel antibodies against viruses in combination with analysis by free zone electrophoresis in a rotating narrow bore tube we have shown that artificial gel antibodies against Semliki Forest Virus (wild type) can sense the difference between this virus and a mutant, although they differ in their chemical composition only by three amino acids in one of the three proteins on the surface of the virus particle. The reason for this extremely high resolution is explained by the fact that we use three types of selectivity: (i) shape selectivity (created by the close fit between the antigen and its imprint in the gel), (ii) bond selectivity in the contact area between the antigen and its imprint in the gel antibody, and (iii) charge selectivity, originating from slightly different structures or/and conformations of the antigens.

Place, publisher, year, edition, pages
2006. Vol. 29, no 18, 2810-2815 p.
Keyword [en]
artificial antibodies, free zone electrophoresis, imprinting, recognition, Semliki Forest Virus
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-146542DOI: 10.1002/jssc.200600212ISI: 000243338800014PubMedID: 17305243OAI: oai:DiVA.org:uu-146542DiVA: diva2:398415
Available from: 2011-02-17 Created: 2011-02-17 Last updated: 2011-02-17Bibliographically approved

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