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Measurements of heme levels and respiration rate in Salmonella enterica serovar typhimurium
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
2010 (English)In: Antibiotic Resistance Protocols / [ed] Gillespie, Stephen H. & McHugh, Timothy D., Humana Press , 2010, 2, Vol. 642, 105-112 p.Chapter in book (Refereed)
Abstract [en]

This chapter describes assays for the measurement of heme levels and the rate of respiration in bacteria. An assay of ALA supplementation is described, in which the effect of exogenous ALA in reversing sensitivity to hydrogen peroxide is an indication of a reduced flow through the heme biosynthesis pathway. A protocol for measurement of the relative amount of heme by a fluorescence assay, based on stripping the iron from the heme moiety, leaving a protoporphyrin molecule which fluoresces following excitation at 400 nm, is also provided. Finally, a method for the measurement of respiration (oxygen consumption) rate is provided. In this method, the respiration of the cell population is expressed as the specific respiration rate during one doubling time of the population.

Place, publisher, year, edition, pages
Humana Press , 2010, 2. Vol. 642, 105-112 p.
Series
Methods in molecular biology, ISSN 1064-3745 ; 642
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-142875DOI: 10.1007/978-1-60327-279-7_8PubMedID: 20401589ISBN: 978-1-60327-278-0 (print)ISBN: 978-1-60327-279-7 (print)OAI: oai:DiVA.org:uu-142875DiVA: diva2:398673
Available from: 2011-02-18 Created: 2011-01-17 Last updated: 2011-07-14Bibliographically approved

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