uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Assays of sensitivity of antibiotic-resistant bacteria to hydrogen peroxide and measurement of catalase activity
National Cancer Institute, NIH, USA.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
2010 (English)In: Antibiotic Resistance Protocols / [ed] Gillespie, Stephen H. & McHugh, Timothy D., Humana Press , 2010, 2, Vol. 642, 95-103 p.Chapter in book (Refereed)
Abstract [en]

Bacteria, in common with other organisms that take advantage of aerobic respiration, generate and accumulate reactive oxygen species (ROS) that damage DNA, fatty acids, and proteins. In addition, intracellular pathogens like Salmonella enterica are exposed to an oxidate burst produced by host macrophages. The relative ability of aerobically growing bacteria to withstand oxidative stress and eliminate ROS has a large impact of their fitness in vitro and in vivo. Methods are described here to measure the viability and relative fitness of bacteria in the presence of hydrogen peroxide. A protocol for the determination of catalase activity, an important part of the ROS detoxification process, is also described.

Place, publisher, year, edition, pages
Humana Press , 2010, 2. Vol. 642, 95-103 p.
Series
Methods in molecular biology, ISSN 1064-3745 ; 642
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-142876DOI: 10.1007/978-1-60327-279-7_7PubMedID: 20401588ISBN: 978-1-60327-278-0 (print)ISBN: 978-1-60327-279-7 (print)OAI: oai:DiVA.org:uu-142876DiVA: diva2:398675
Available from: 2011-02-18 Created: 2011-01-17 Last updated: 2011-07-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Hughes, Diarmaid

Search in DiVA

By author/editor
Hughes, Diarmaid
By organisation
Microbiology
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
isbn
urn-nbn

Altmetric score

doi
pubmed
isbn
urn-nbn
Total: 666 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf