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Peripheral administration of pancreatic polypeptide inhibits components of food- intake behavior in dogs
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Pharmacology.
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2010 (English)In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 31, no 6, 1055-1061 p.Article in journal (Refereed) Published
Abstract [en]

Pancreatic polypeptide (PP) belongs to the neuropeptide Y (NPY) family of peptides and is released from pancreatic F cells postprandially. PP functions as a peptide hormone and has been associated with decreased food intake in humans and rodents. Our study describes the effects of PP on feeding behavior in dogs, whose mammalian order (Carnivora) is more distantly related to primates and rodents than these are to each other. Furthermore, obesity is becoming more prevalent in dogs which makes knowledge about their appetite regulation highly relevant. Repeated peripheral administration of physiological doses of PP (three injections of 30 pmol/kg each that were administered within 30 min) to six male beagle dogs prolonged the median time spent eating three servings of food by 19% but resulted in no reduction of food intake. In addition, PP decreased the duration of food-seeking behavior after the first serving by 71%. Thus, a physiological dose of PP seems to decrease both the appetitive and the consummatory drive in dogs.

Place, publisher, year, edition, pages
2010. Vol. 31, no 6, 1055-1061 p.
Keyword [en]
Pancreatic polypeptide, Appetite, Satiety, Feeding, Food intake, Dog
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-147123DOI: 10.1016/j.peptides.2010.03.019ISI: 000283939000009PubMedID: 20338207OAI: oai:DiVA.org:uu-147123DiVA: diva2:399899
Available from: 2011-02-24 Created: 2011-02-24 Last updated: 2017-12-11Bibliographically approved
In thesis
1. Functional Studies of the Neuropeptide Y System: Receptor-Ligand Interaction and Regulation of Food Intake
Open this publication in new window or tab >>Functional Studies of the Neuropeptide Y System: Receptor-Ligand Interaction and Regulation of Food Intake
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The members of the mammalian neuropeptide Y family, i.e. the peptides neuropeptide Y (NPY), peptide YY (PYY) and pancreatic polypeptide (PP), are all involved in regulation of food intake. In human and most other mammals they act via receptors Y1, Y2, Y4 and Y5. NPY is released in the hypothalamus and is one of the strongest appetite-stimulating neurotransmitters whereas PP and PYY are secreted from gut endocrine cells after meals and function as appetite-reducing hormones. This thesis describes studies of the NPY system at both the molecular and the physiological level. The first part describes two investigations of receptor-ligand interactions with the human Y1 and Y2 receptors. The results clarify the importance of several amino-acid residues of the human Y1 receptor. Three amino acids previously suggested by others to form a binding pocket for the carboxy-terminus of the peptide were confirmed to be crucial for interaction with peptide ligands. However, they were found to be too distantly located from each other to be able to form a binding pocket. Further investigation of the three corresponding positions in the human Y2 receptor showed that only one of the positions was important for interaction with full-length peptides. The results indicate overlapping but, surprisingly, non-identical binding of the different peptides to human Y1 and Y2 receptors, despite the fact that the two receptors share a common ancestor. The second part of the thesis describes an investigation of the effect of PP on food intake in six beagle dogs and a test for personality characteristics in dogs (TFPC). Treatment with physiological doses of PP decreased both the appetitive and the consummatory drive but had no effect on the amount food consumed. The TFPC protocol was used to map individual behavioral differences in a population of sixteen beagle dogs. The test, which included several situations that may appear in an experimental study, revealed considerable inter-individual differences in behavioral responses despite the fact that the dogs were born and housed in the same animal facility in constant controlled conditions. These results demonstrate that PP can influence food intake in distantly related mammals and emphasize the importance of considering differences in personality in experimental animals.

Place, publisher, year, edition, pages
Uppsala: Universitetsbiblioteket, 2009. 67 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 415
Keyword
neuropeptide Y, G-protein coupled receptor (GPCR), site-directed mutagenesis, pancreatic polypeptide, food intake, dog
National Category
Pharmacology and Toxicology
Identifiers
urn:nbn:se:uu:diva-9533 (URN)978-91-554-7393-8 (ISBN)
Public defence
2009-02-20, B21, BMC, Husargatan 3, Uppsala, 13:15
Opponent
Supervisors
Available from: 2009-01-30 Created: 2009-01-30 Last updated: 2013-01-07Bibliographically approved

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