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Biomimetic calcium phosphate coatings on recombinant spider silk fibres
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Experimental Physics.
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2010 (English)In: Biomedical Materials, ISSN 1748-6041, Vol. 5, no 4, 045002- p.Article in journal (Refereed) Published
Abstract [en]

Calcium phosphate ceramic coatings, applied on surfaces of metallic and polymeric biomaterials, can improve their performance in bone repair and regeneration. Spider silk is biocompatible, strong and elastic, and hence an attractive biomaterial for applications in connective tissue repair. Recently, artificial spider silk, with mechanical and structural characteristics similar to those of native spider silk, has been produced from recombinant minispidroins. In the present study, supersaturated simulated body fluid was used to deposit calcium phosphate coatings on recombinant spider silk fibres. The mineralization process was followed in time using scanning electron microscopy equipped with an energy dispersive x-ray (EDX) detector and Raman spectroscope. Focused ion beam technology was used to produce a cross section of a coated fibre, which was further analysed by EDX. Preliminary in vitro experiments using a culture of bone marrow-derived human mesenchymal stem cells (hMSCs) on coated fibres were also performed. This study showed that recombinant spider silk fibres were successfully coated with a homogeneous and thick crystalline calcium phosphate layer. In the course of the mineralization process from modified simulated body fluid, sodium chloride crystals were first deposited on the silk surface, followed by the deposition of a calcium phosphate layer. The coated silk fibres supported the attachment and growth of hMSCs.

Place, publisher, year, edition, pages
2010. Vol. 5, no 4, 045002- p.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-147276DOI: 10.1088/1748-6041/5/4/045002ISI: 000280580900014PubMedID: 20539057OAI: oai:DiVA.org:uu-147276DiVA: diva2:400124
Available from: 2011-02-24 Created: 2011-02-24 Last updated: 2016-04-19Bibliographically approved

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Blom, TobiasLeifer, Klaus

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