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A microsphere-based immunoassay for rapid and sensitive detection of bovine viral diarrhoea virus antibodies
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2010 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 168, no 1-2, 18-21 p.Article in journal (Refereed) Published
Abstract [en]

This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes.

Place, publisher, year, edition, pages
2010. Vol. 168, no 1-2, 18-21 p.
Keyword [en]
Luminex, Microsphere immunoassay, BVDV
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-147273DOI: 10.1016/j.jviromet.2010.04.009ISI: 000280869400004PubMedID: 20403384OAI: oai:DiVA.org:uu-147273DiVA: diva2:400146
Available from: 2011-02-24 Created: 2011-02-24 Last updated: 2017-12-11Bibliographically approved

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