Powerful protein binders from designed polypeptides and small organic molecules: a general concept for protein recognition
2011 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 50, no 8, 1823-1827 p.Article in journal (Refereed) Published
High-affinity binders for the C-reactive protein (CRP), with dissociation constants in the pM to nM range and selectivities in human serum comparable to those of antibodies, were obtained by conjugation of 16 designed polypeptides to phosphocholine, a small molecule that binds CRP with a KDvalue of 5I . The polypeptides were not designed specifically to recognize CRP and bind by an adapted fit mechanism.
Place, publisher, year, edition, pages
2011. Vol. 50, no 8, 1823-1827 p.
bioorganic chemistry, C-reactive protein, phosphocholine, polypeptides, proteins
Chemical Sciences Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-147682DOI: 10.1002/anie.201005059ISI: 000287836200012PubMedID: 21328648OAI: oai:DiVA.org:uu-147682DiVA: diva2:400645