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Novel means of viral antigen identification: Improved detection of avian influenza viruses by proximity ligation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2010 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 163, no 1, 116-122 p.Article in journal (Refereed) Published
Abstract [en]

Recent outbreaks of avian influenza in different parts of the world have caused major economic losses for the poultry industry, affected wildlife seriously and present a significant threat even to human public health, due to the risk for zoonotic transmission. The ability to recognize avian influenza viruses (AIVs) early is of paramount importance to ensure that appropriate measures can be taken quickly to contain the outbreak. In this study. the performance of a proximity ligation assay (PLA) for the detection of AIV antigens in biological specimens was evaluated. It is shown that PLA: (i) as a novel principle of highly sensitive antigen detection is extending the arsenal of tools for the diagnosis of AIV; (ii) is very specific, nearly as sensitive as a commonly used reference real-time PCR assay, and four orders of magnitude more sensitive than a sandwich ELISA, utilizing the same antibody; (iii) avoids the necessity of nucleic acids extraction, which greatly facilitates high-throughput implementations; (iv) allows the use of inactivated samples, which safety can be transported from the field to diagnostic laboratories for further analysis. In summary, the results demonstrate that PLA is suited for rapid, accurate and early detection of AIV.

Place, publisher, year, edition, pages
2010. Vol. 163, no 1, 116-122 p.
Keyword [en]
Infectious disease diagnosis, Proximity ligation, PCR, Monoclonal antibodies, Biotinylation, Avian influenza
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-147707DOI: 10.1016/j.jviromet.2009.09.008ISI: 000274042900016PubMedID: 19751768OAI: oai:DiVA.org:uu-147707DiVA: diva2:400738
Available from: 2011-02-28 Created: 2011-02-28 Last updated: 2017-12-11Bibliographically approved

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