The effect of substance P1-7 amide on nociceptive threshold in diabetic mice
2011 (English)In: Peptides, ISSN 0196-9781, E-ISSN 1873-5169, Vol. 32, no 1, 93-98 p.Article in journal (Refereed) Published
We previously demonstrated that intrathecal treatment with substance P metabolite substance P1-7 induced anti-hyperalgesia in diabetic mice. In the present study, we have used a synthetic analog of this peptide, the substance P1-7 amide, showing higher binding affinitiy than the native heptapeptide, for studies of the tail-flick response in diabetic and non-diabetic mice. Intrathecal injection of substance P1-7 amide produced prolongation of the tail-flick latency in both diabetic and non-diabetic mice, an effect that was more pronounced in diabetic mice than non-diabetic mice. Moreover, the observed antinociceptive potency of the substance P1-7 amide was higher in both diabetic and non-diabetic mice in comparison with the native substance P1-7. The antinociceptive effect of substance P1-7 amide was reversed by naloxone but not by the selective opioid receptor antagonist beta-funaltrexamine, naltrindole or nor-binaltorphimine, selective for the mu-, delta- or kappa-opioid receptor, respectively. In addition, the antinociceptive effect induced by substance P1-7 amide was partly reversed by the sigma(1) receptor agonist (+)-pentazocine, suggesting a possible involvement of the sigma(1) receptor for the action of this peptide. These results suggest that the actions of substance P1-7 amide mimic the effects of the native substance P fragment but with higher potency and that the mechanisms for its action may involve the sigma(1) receptor system.
Place, publisher, year, edition, pages
2011. Vol. 32, no 1, 93-98 p.
Antinociception, Diabetes, Substance P fragment substance P1-7, Opioid receptors, Substance P1-7 amide, Sigma receptor
IdentifiersURN: urn:nbn:se:uu:diva-147780DOI: 10.1016/j.peptides.2010.09.029ISI: 000286562300015PubMedID: 20933559OAI: oai:DiVA.org:uu-147780DiVA: diva2:400846