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Functional interactions in vivo between suppressor tRNA and mutationally altered ribosomal protein S4
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology. (Kirsebom)
1986 (English)In: Molecular General Genetics, ISSN 0026-8925, E-ISSN 1432-1874, Vol. 205, no 2, 240-247 p.Article in journal (Refereed) Published
Abstract [en]

Ribosomal mutants (rpsD) which are associated with a generally increased translational ambiguity were investigated for their effects in vivo on individual tRNA species using suppressor tRNAs as models. It was found that nonsense suppression is either increased, unaffected or decreased depending on the codon context and the rpsD allele involved as well as the nature of the suppressor tRNA. Missense suppression of AGA and AGG by glyT(SuAGA/G) tRNA as well as UGG by glyT(SuUGG-8) tRNA is unaffected whereas suppression of UGG by glyT(SuUGA/G) or glyV(SuUGA/G) tRNA is decreased in the presence of an rpsD mutation. The effects on suppressor tRNA are thus not correlated with the ribosomal ambiguity (Ram) phenotype of the rpsD mutants used in this study. It is suggested that the mutationally altered ribosomes are changed in functional interactions with the suppressor tRNA itself rather than with the competing translational release factor(s) or cognate aminoacyl tRNA. The structure of suppressor tRNA, particularly the anticodon loop, and the suppressed codon as well as the codon context determine the allele specific functional interactions with these ribosomal mutations.

Place, publisher, year, edition, pages
1986. Vol. 205, no 2, 240-247 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-147812DOI: 10.1007/BF00430434PubMedID: 3543619OAI: oai:DiVA.org:uu-147812DiVA: diva2:400886
Available from: 2011-02-28 Created: 2011-02-28 Last updated: 2011-11-30Bibliographically approved

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