Mutations in ribosomal proteins L7/L12 perturb EF-G and EF-Tu functons
1988 (English)In: Biochimie, ISSN 0300-9084, E-ISSN 1638-6183, Vol. 70, no 5, 611-618 p.Article in journal (Refereed) Published
In vitro cycling rates of E. coli ribosomes and of elongation factors EF-Tu and EF-G have been obtained and these are compatible with translation rates in vivo. We show that the rate of translocation is faster than 50 s-1 and therefore that the EF-G function is not a rate limiting step in protein synthesis. The in vivo phenotype of some L7/L12 mutants could be accounted for by perturbed EF-Tu as well as EF-G functions. The S12 mutants that we studied were, in contrast, only perturbed in their EF-Tu function, while their EF-G interaction was not impaired in relation to wild type ribosomes.
Place, publisher, year, edition, pages
1988. Vol. 70, no 5, 611-618 p.
ribosomal mutants, in vitro translation
IdentifiersURN: urn:nbn:se:uu:diva-147814DOI: 10.1016/0300-9084(88)90244-1PubMedID: 3139080OAI: oai:DiVA.org:uu-147814DiVA: diva2:400887