Studies on the interactions between glycosylated beta(3)-peptides and the lectin Vicia villosa by saturation transfer difference NMR spectroscopy
2009 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 344, no 18, 2577-2580 p.Article in journal (Refereed) Published
Saturation transfer difference (STD) NMR spectroscopy was used to study the interaction of the lectin Vicia villosa (VVLB4) with alpha-D-GalNAc glycosylated beta(3)-peptides. The data were compared to those obtained with the monosaccharides D-Gal, D-GalNAc, and D-Glc as well as with those obtained with the Tn antigen alpha-glycopeptide (D-GalNAc-alpha-O-Ser/Thr), molecule naturally recognized by V. villosa. Evidence that the lectin also recognizes glycosylated beta(3)-peptides and has close contact with both the sugar and amino acid moieties was obtained.
Place, publisher, year, edition, pages
2009. Vol. 344, no 18, 2577-2580 p.
STD NMR, Glycosylated beta(3)-peptides, Tn antigen, Lectin Vicia villosa (isolectin B-4)
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-148210DOI: 10.1016/j.carres.2009.06.040ISI: 000272860500022PubMedID: 19863951OAI: oai:DiVA.org:uu-148210DiVA: diva2:401645