Image Based Measurements of Single Cell mtDNA Mutation Load
2007 (English)In: Image Analysis, Proceedings / [ed] Ersboll BK, Pedersen KS, 2007, 631-640 p.Conference paper (Refereed)
Cell cultures as well as cells in tissue always display a certain degree of variability, and measurements based on cell averages will miss important information contained in a heterogeneous population. This paper presents automated methods for image based measurements of mitochondiral DNA (mtDNA) mutations in individual cells. The mitochondria are present in the cell’s cytoplasm, and each cytoplasm has to be delineated. Three different methods for segmentation of cytoplasms are compared and it is shown that automated cytoplasmic delineation can be performed 30 times faster than manual delineation, with an accuracy as high as 87%. The final image based measurements of mitochondrial mutation load are also compared to, and show high agreement with, measurements made using biochemical techniques.
Place, publisher, year, edition, pages
2007. 631-640 p.
, Lecture notes in computer science, ISSN 0302-9743 ; 4522
single cell analysis, cytoplasm segmentation, mitochondrial DNA, image cytometry
Computer Vision and Robotics (Autonomous Systems)
IdentifiersURN: urn:nbn:se:uu:diva-12453DOI: 10.1007/978-3-540-73040-8ISI: 000247364000064ISBN: 978-3-540-73039-2OAI: oai:DiVA.org:uu-12453DiVA: diva2:40222
15th Scandinavian Conference, SCIA 2007, Aalborg, Denmark, June 10-14, 2007