Adsorption behaviour of a quinidine carbamate-based chiral stationary phase: Role of the additive
2009 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1216, no 16, 3480-3487 p.Article in journal (Refereed) Published
In this study, we incorporate the additive properties into the theoretical model of a general preparative chromatographic system; this is normally not done and this limits a proper process optimization. As a model phase system, we used the adsorption of 9H-fluoren-9-ylmethoxycarbonyl-allylglycine (Fmoc-allylglycine) enantiomers on a quinidine carbamate-based chiral stationary phase (anion exchanger) together with a methanol-glacial acetic acid-ammonium acetate eluent. The inverse method was used to measure the competitive adsorption isotherms of both the Fmoc-allylglycine enantiomers as well as the non-detectable additive acetic acid. It was concluded that this enantioselective preparative system is well described by a non-heterogeneous adsorption model and that the loading capacity is very high. The proposed model is valid over a wide range of additive concentrations, which is important for process optimization.
Place, publisher, year, edition, pages
2009. Vol. 1216, no 16, 3480-3487 p.
Chiral stationary phase, Quinidine carbamate-based chiral stationary phase, Chiral preparative chromatography, Adsorption isotherm data, Inverse method, Loadability
IdentifiersURN: urn:nbn:se:uu:diva-148884DOI: 10.1016/j.chroma.2008.11.039ISI: 000265262300008PubMedID: 19062026OAI: oai:DiVA.org:uu-148884DiVA: diva2:403456