uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Genomic Stability over 9 Years of an Isoniazid Resistant Mycobacterium tuberculosis Outbreak Strain in Sweden
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Show others and affiliations
2011 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 6, no 1, e16647- p.Article in journal (Refereed) Published
Abstract [en]

In molecular epidemiological studies of drug resistant Mycobacterium tuberculosis (TB) in Sweden a large outbreak of an isoniazid resistant strain was identified, involving 115 patients, mainly from the Horn of Africa. During the outbreak period, the genomic pattern of the outbreak strain has stayed virtually unchanged with regard to drug resistance, IS6110 restriction fragment length polymorphism and spoligotyping patterns. Here we present the complete genome sequence analyses of the index isolate and two isolates sampled nine years after the index case as well as experimental data on the virulence of this outbreak strain. Even though the strain has been present in the community for nine years and passaged between patients at least five times in-between the isolates, we only found four single nucleotide polymorphisms in one of the later isolates and a small (4 amino acids) deletion in the other compared to the index isolate. In contrast to many other evolutionarily successful outbreak lineages (e. g. the Beijing lineage) this outbreak strain appears to be genetically very stable yet evolutionarily successful in a low endemic country such as Sweden. These findings further illustrate that the rate of genomic variation in TB can be highly strain dependent, something that can have important implications for epidemiological studies as well as development of resistance.

Place, publisher, year, edition, pages
2011. Vol. 6, no 1, e16647- p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-148990DOI: 10.1371/journal.pone.0016647ISI: 000286834300107PubMedID: 21304944OAI: oai:DiVA.org:uu-148990DiVA: diva2:403669
Available from: 2011-03-14 Created: 2011-03-14 Last updated: 2011-06-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Sandegren, Linus
By organisation
Department of Medical Biochemistry and Microbiology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 239 hits
ReferencesLink to record
Permanent link

Direct link