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GAD1 is a biomarker for benign and malignant prostatic tissue
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular and Morphological Pathology. (Forskargrupp Pontén)
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2011 (English)In: Scandinavian Journal of Urology and Nephrology, ISSN 0036-5599, E-ISSN 1651-2065, Vol. 45, no 1, 39-45 p.Article in journal (Refereed) Published
Abstract [en]

Objective. Tissue-specific markers are useful for identification of tumour type in advanced cancers of unknown origin. This study investigated the expression of glutamate decarboxylase 1 (GAD1) in prostate and control tissue compared with the established prostate-specific markers prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSMA). Material and methods. A tissue microarray was constructed of 36 prostate adenocarcinomas, eight benign prostate samples and benign and malignant control tissues from urinary bladder, lung and rectum. Immunohistochemistry for GAD 1, PSA and PSMA was performed. The products of staining intensity and extent were analysed. The GAD1 antibody was validated by Western blot. Real-time polymerase chain reaction (RT-PCR) was performed on malignant and benign samples from each tissue type. Results. GAD 1 and PSA immunostains were significantly stronger in malignant and benign prostatic tissue than in controls. PSMA was stronger in prostate cancer than in urothelial and rectal cancer but had a lower specificity than GAD1 and PSA. GAD I expression decreased with increasing Gleason score. RT-PCR confirmed the presence of mRNA for GAD I, PSA and PSMA in prostate samples. Conclusion. GAD1 is expressed in benign and malignant prostatic tissue and may serve as a highly prostate-specific tissue biomarker.

Place, publisher, year, edition, pages
2011. Vol. 45, no 1, 39-45 p.
Keyword [en]
GAD1, immunohistochemisny, prostate, prostate cancer, tissue microarray
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-148985DOI: 10.3109/00365599.2010.521189ISI: 000287123700006PubMedID: 21091088OAI: oai:DiVA.org:uu-148985DiVA: diva2:403677
Available from: 2011-03-14 Created: 2011-03-14 Last updated: 2011-03-14Bibliographically approved

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